Multimedia Collection

Polymerase Chain Reaction (PCR) and Detection

VHS
30 minutes
QP606 .D46 P61 1991

The explosion of scientific discovery has been facilitated by the polymerase chain reaction (PCR). The process of amplifying DNA of interest about one-billion-fold by PCR is documented in this video. Principles of PCR are illustrated with animation. Precautions are used to avoid contamination, a potentially serious problem with inexperienced investigators. Optimization of PCR and three methods for detecting PCR amplification products, including oligomer hybridization, are outlined.

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Biology

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