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Published Papers
| Independent Functions of Viral Protein and Nucleic Acid in Growth of Bacteriophage. September 20, 1952. |
Page 12 [50]
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Authors: Martha Chase, Alfred Hershey
![Page 12 [50]](hersheychase-pg12-xl.jpg "Page 12 [50]")
Page 12 [50]
| Title: |
Independent Functions of Viral Protein and Nucleic Acid in Growth of Bacteriophage [12 of 18] |
| Creator: |
Chase, Martha |
| Contributor: |
Hershey, Alfred Day, 1908 |
| Publisher: |
Journal of General Physiology |
| Date: |
1952-09-20 |
| Subject: |
Molecular biology
Molecular genetics
|
| Description: |
From the Journal of General Physiology Vol. 36, No. 1. |
| Type: |
Text |
| Format: |
text/plain |
| Language: |
en |
| Identifier: |
hersheychase-pg12.jpg |
| Source: |
Master scanned with Epson GT-10000+ flatbed scanner at 600 dpi. |
| Rights: |
http://osulibrary.orst.edu/specialcollections/coll/pauling/dna/copyright.html |
| Full Text: |
50 VIRAL PROTEIN AND NUCLEIC ACID IN BACTERIOPHAGE GROWTH
The distribution of S35 and phage among fractions obtained from three cul-
tures of this kind is shown in Table %'I. The results are typical (except for the
excessively good recoveries of phage and Sab) of lysates in broth as well as
lysates in glycerol-lactate medium.
The striking result of this experiment is that the distribution of S'S among
the fractions is the same for early lysates that do not contain phage progeny,
and later ones that do. This suggests that little or no S'° is contained in the
mature phage progeny. Further fractionation by adsorption to bacteria con-
firms this suggestion.
Adsorption mixtures prepared for this purpose contained about 5 X 10'
heat-killed bacteria (70°C. for 10 minutes) from 18 hour broth cultures, and
TABLE VI
Per Cent Distributions of Phage and S26 among Cenlrifugally Separated Fractions of Lysales
after Infection with S15-Labeled T2
Lysis at Lysis at I Maximal yield
Fraction I t = 0 l = 10 ..-_ __
__
SOS Sas
S16 Phage
1st low speed sediment....... . . . . . . . ....... . . 79 81 82 1 19
2nd « « « ...................... 2.4 2.1 2.8 ( 14
High speed ... . ..... 8.6 6.9 7.1 61
11 supernatant........... .. ....... 10 10 I, 7.5 7.0
_-
Recovery............ ....,. .. ...... . ...I 100 100 96 100
Infection with S'6-laheled T2, 0.8 particles per bacterium. Lysing phage UV-killed h
mutant of T2. Phage yields per infected bacterium: <0.1 after lysis at t = 0; 0.12 at t =
10; maximal yield 29. Recovery of S06 means per cent of adsorbed input recovered in the
four fractions; recovery of phage means per cent of total phage yield (by plaque count before
fractionation) recovered by titration of fractions.
about 1011 phage (UV-killed lysing phage plus test phage), per ml. of adsorp-
tion medium. After warming to 37°C. for 5 minutes, the mixtures were diluted
with 2 volumes of water, and centrifuged. Assays were made from supernatants
and from unwashed resuspended sediments.
The results of tests of adsorption of S" and phage to bacteria (H) adsorbing
both T2 progeny and h-mutant lysing phage, to bacteria (B/2) adsorbing lysing
phage only, and to bacteria (B 2k) adsorbing neither, are shown in Table VII,
together with parallel tests of authentic S'1-labeled phage.
The adsorption tests show that the S35 present in the seed phage is adsorbed
with the specificity of the phage, but that S'S present in lysates of bacteria
infected with this phage shows a more complicated behavior. It is strongly
adsorbed to bacteria adsorbing both progeny and lysing phage. It is weakly
adsorbed to bacteria adsorbing neither. It is moderately well adsorbed to bac-
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